Endoscopic detection of murine colonic dysplasia using a novel fluorescence-labeled peptide

Sharon J. Miller; Bishnu P. Joshi; Adam Gaustad; Eric R. Fearon; Thomas D. Wang

doi:10.1117/12.873530

22 February 2011 Endoscopic detection of murine colonic dysplasia using a novel fluorescence-labeled peptide

Sharon J. Miller, Bishnu P. Joshi, Adam Gaustad, Eric R. Fearon, Thomas D. Wang

Proceedings Volume 7893, Endoscopic Microscopy VI; 789303 (2011) https://doi.org/10.1117/12.873530
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Current endoscopic screening does not detect all pre-malignant (dysplastic) colorectal mucosa, thus requiring the development of more sensitive, targeted techniques to improve detection. The presented work utilizes phage display to identify a novel peptide binder to colorectal dysplasia in a CPC;Apc mouse model. A wide-field, small animal endoscope capable of fluorescence excitation (450-475 nm) identified polyps via white light and also collected fluorescence images (510 nm barrier filter) of peptide binding. The peptide bound ~2-fold greater to the colonic adenomas when compared to the control peptide. We have imaged fluorescence-labeled peptide binding in vivo that is specific towards distal colonic adenomas.

Citation Download Citation

Sharon J. Miller, Bishnu P. Joshi, Adam Gaustad, Eric R. Fearon, and Thomas D. Wang "Endoscopic detection of murine colonic dysplasia using a novel fluorescence-labeled peptide", Proc. SPIE 7893, Endoscopic Microscopy VI, 789303 (22 February 2011); https://doi.org/10.1117/12.873530

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KEYWORDS

Endoscopy

In vivo imaging

Tissues

Colon

Luminescence

Image filtering

Mouse models

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