Dynamics of hybrid amoeba proteus containing zoochlorellae studied using fluorescence spectroscopy

C.-H. Liu; B. A. Fong; S. A. Alfano Jr.; I. Rakhlin; W. B. Wang; X. H. Ni; Y. L. Yang; F. Zhou; R. C. Zuzolo; R. R. Alfano

doi:10.1117/12.875293

16 February 2011 Dynamics of hybrid amoeba proteus containing zoochlorellae studied using fluorescence spectroscopy

C.-H. Liu, B. A. Fong, S. A. Alfano Jr., I. Rakhlin, W. B. Wang, X. H. Ni, Y. L. Yang, F. Zhou, R. C. Zuzolo, R. R. Alfano

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Proceedings Volume 7895, Optical Biopsy IX; 78950Y (2011) https://doi.org/10.1117/12.875293
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The microinjection of organelles, plants, particles or chemical solutions into Amoeba proteus coupled with spectroscopic analysis and observed for a period of time provides a unique new model for cancer treatment and studies. The amoeba is a eukaryote having many similar features of mammalian cells. The amoeba biochemical functions monitored spectroscopically can provide time sequence in vivo information about many metabolic transitions and metabolic exchanges between cellar organelles and substances microinjected into the amoeba. It is possible to microinject algae, plant mitochondria, drugs or carcinogenic solutions followed by recording the native fluorescence spectra of these composites. This model can be used to spectroscopically monitor the pre-metabolic transitions in developing diseased cells such as a cancer. Knowing specific metabolic transitions could offer solutions to inhibit cancer or reverse it as well as many other diseases. In the present study a simple experiment was designed to test the feasibility of this unique new model by injecting algae and chloroplasts into amoeba. The nonradiative dynamics found from these composites are evidence in terms of the emission ratios between the intensities at 337nm and 419nm; and 684nm bands. There were reductions in the metabolic and photosynthetic processes in amoebae that were microinjected with chloroplasts and zoochlorellae as well of those amoebae that ingested the algae and chloroplasts. The changes in the intensity of the emissions of the peaks indicate that the zoochlorellae lived in the amoebae for ten days. Spectral changes in intensity under the UV and 633nm wavelength excitation are from the energy transfer of DNA and RNA, protein-bound chromophores and chlorophylls present in zoochlorellae undergoing photosynthesis. The fluorescence spectroscopic probes established the biochemical interplay between the cell organelles and the algae present in the cell cytoplasm. This hybrid state is indicative that a symbiotic system is in place and the results definitely support the potential use of this unique new model. This model many help in plant / animal and cancer processes.

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C.-H. Liu, B. A. Fong, S. A. Alfano Jr., I. Rakhlin, W. B. Wang, X. H. Ni, Y. L. Yang, F. Zhou, R. C. Zuzolo, and R. R. Alfano "Dynamics of hybrid amoeba proteus containing zoochlorellae studied using fluorescence spectroscopy", Proc. SPIE 7895, Optical Biopsy IX, 78950Y (16 February 2011); https://doi.org/10.1117/12.875293

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KEYWORDS

Luminescence

Spectroscopy

Photosynthesis

Fluorescence spectroscopy

Proteins

Cancer

Chromophores

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