Vibrational spectroscopy characterization of low level laser therapy on mammary culture cells: a micro-FTIR study

Taciana D. Magrini; Nathalia Villa dos Santos; Marcella Pecora Milazzotto; Giselle Cerchiaro; Herculano da Silva Martinho

doi:10.1117/12.875445

18 February 2011 Vibrational spectroscopy characterization of low level laser therapy on mammary culture cells: a micro-FTIR study

Taciana D. Magrini, Nathalia Villa dos Santos, Marcella Pecora Milazzotto, Giselle Cerchiaro, Herculano da Silva Martinho

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Proceedings Volume 7897, Optical Interactions with Tissue and Cells XXII; 78970T (2011) https://doi.org/10.1117/12.875445
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Low level laser therapy (LLLT) is an emerging therapeutic approach for several clinical conditions. The clinical effects induced by LLLT presumably go from the photobiostimulation/photobioinibition at cellular level to the molecular level. The detailed mechanism underlying this effect is still obscure. This work is dedicated to quantify some relevant aspects of LLLT related to molecular and cellular variations. This goal was attached by exposing malignant breast cells (MCF7) to spatially filtered light of a He-Ne laser (633 nm) with 28.8 mJ/cm² of fluency. The cell viability was evaluated by microscopic observation using Trypan Blue viability test. The vibrational spectra of each experimental group (micro- FTIR technique) were used to identify the relevant biochemical alterations occurred due the process. The red light had influence over RNA, phosphate and serine/threonine/tyrosine bands. Light effects on cell number or viability were not detected. However, the irradiation had direct influence on metabolic activity of cells.

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Taciana D. Magrini, Nathalia Villa dos Santos, Marcella Pecora Milazzotto, Giselle Cerchiaro, and Herculano da Silva Martinho "Vibrational spectroscopy characterization of low level laser therapy on mammary culture cells: a micro-FTIR study", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970T (18 February 2011); https://doi.org/10.1117/12.875445

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KEYWORDS

Laser therapeutics

Proteins

FT-IR spectroscopy

Spectroscopy

Acquisition tracking and pointing

Phototherapy

Helium neon lasers

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