Laser injury and in vivo multimodal imaging using a mouse model

Ginger M. Pocock; Adam Boretsky; Praveena Gupta; Jeff W. Oliver; Massoud Motamedi

doi:10.1117/12.873979

17 February 2011 Laser injury and in vivo multimodal imaging using a mouse model

Ginger M. Pocock, Adam Boretsky, Praveena Gupta, Jeff W. Oliver, Massoud Motamedi

Proceedings Volume 7897, Optical Interactions with Tissue and Cells XXII; 789713 (2011) https://doi.org/10.1117/12.873979
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Balb/c wild type mice were used to perform in vivo experiments of laser-induced thermal damage to the retina. A Heidelberg Spectralis HRA confocal scanning laser ophthalmoscope with a spectral domain optical coherence tomographer was used to obtain fundus and cross-sectional images of laser induced injury in the retina. Sub-threshold, threshold, and supra-threshold lesions were observed using optical coherence tomography (OCT), infrared reflectance, red-free reflectance, fluorescence angiography, and autofluorescence imaging modalities at different time points post-exposure. Lesions observed using all imaging modalities, except autofluorescence, were not visible immediately after exposure but did resolve within an hour and grew in size over a 24 hour period. There was a decrease in fundus autofluorescence at exposure sites immediately following exposure that developed into hyper-fluorescence 24-48 hours later. OCT images revealed threshold damage that was localized to the RPE but extended into the neural retina over a 24 hour period. Volumetric representations of the mouse retina were created to visualize the extent of damage within the retina over a 24 hour period. Multimodal imaging provides complementary information regarding damage mechanisms that may be used to quantify the extent of the damage as well as the effectiveness of treatments without need for histology.

Citation Download Citation

Ginger M. Pocock, Adam Boretsky, Praveena Gupta, Jeff W. Oliver, and Massoud Motamedi "Laser injury and in vivo multimodal imaging using a mouse model", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 789713 (17 February 2011); https://doi.org/10.1117/12.873979

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