Depth-resolved optical imaging of hemodynamic response in mouse brain with microcirculatory beds

Yali Jia; Rosemary Nettleton; Mara Rosenberg; Eilis Boudreau; Ruikang K. Wang

doi:10.1117/12.874232

28 February 2011 Depth-resolved optical imaging of hemodynamic response in mouse brain with microcirculatory beds

Yali Jia, Rosemary Nettleton, Mara Rosenberg, Eilis Boudreau, Ruikang K. Wang

Proceedings Volume 7898, Dynamics and Fluctuations in Biomedical Photonics VIII; 789812 (2011) https://doi.org/10.1117/12.874232
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Optical hemodynamic imaging employed in pre-clinical studies with high spatial and temporal resolution is significant to unveil the functional activities of brain and the mechanism of internal or external stimulus effects in diverse pathological conditions and treatments. Most current optical systems only resolve hemodynamic changes within superficial macrocirculatory beds, such as laser speckle contrast imaging; or only provide vascular structural information within microcirculatory beds, such as multi-photon microscopy. In this study, we introduce a hemodynamic imaging system based on Optical Micro-angiography (OMAG) which is capable of resolving and quantifying 3D dynamic blood perfusion down to microcirculatory level. This system can measure the optical phase shifts caused by moving blood cells in microcirculation. Here, the utility of OMAG was demonstrated by monitoring the hemodynamic response to alcohol administration in mouse prefrontal cortex. Our preliminary results suggest that the spatiotemporal tracking of cerebral micro-hemodynamic using OMAG can be successfully applied to the mouse brain and reliably distinguish between vehicle and alcohol stimulation experiment.

Citation Download Citation

Yali Jia, Rosemary Nettleton, Mara Rosenberg, Eilis Boudreau, and Ruikang K. Wang "Depth-resolved optical imaging of hemodynamic response in mouse brain with microcirculatory beds", Proc. SPIE 7898, Dynamics and Fluctuations in Biomedical Photonics VIII, 789812 (28 February 2011); https://doi.org/10.1117/12.874232

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