Two channel microfluidic CARS for quantifying pure vibrational contrast of model analytes

B. Dietzek; G. Bergner; D. Akimov; D. Malsch; T. Henkel; S. Schlücker; J. Popp

doi:10.1117/12.853843

28 April 2010 Two channel microfluidic CARS for quantifying pure vibrational contrast of model analytes

B. Dietzek, G. Bergner, D. Akimov, D. Malsch, T. Henkel, S. Schlücker, J. Popp

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77152X (2010) https://doi.org/10.1117/12.853843
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

We discuss the combination of a CARS-imaging system with microfluidics. Such system is a versatile tool to quantify the relative contributions of resonant and non-resonant scattering at the CARS frequency. We will show that the twochannel microfluidic chip employed in combination with deuterated isotopomers as an internal standard allows for fast and quantitative detection of organic molecules by CARS microscopy. The experimental design enables the simultaneous measurement of both the chemically relevant Raman-resonant signal and the non-Raman-resonant background.

Citation Download Citation

B. Dietzek, G. Bergner, D. Akimov, D. Malsch, T. Henkel, S. Schlücker, and J. Popp "Two channel microfluidic CARS for quantifying pure vibrational contrast of model analytes", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77152X (28 April 2010); https://doi.org/10.1117/12.853843

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