DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Monika Rutowska; Jiadi Lu; Fatima C. Garcia-Gunning; Andrew D. Ellis

doi:10.1117/12.886070

17 May 2011 DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre

Monika Rutowska, Jiadi Lu, Fatima C. Garcia-Gunning, Andrew D. Ellis

Proceedings Volume 7753, 21st International Conference on Optical Fiber Sensors; 77536I (2011) https://doi.org/10.1117/12.886070
Event: 21st International Conference on Optical Fibre Sensors (OFS21), 2011, Ottawa, Canada

Abstract

In this paper, we report for the first time the detection of a Cy5-labelled DNA probe immobilised within a 3D hydrogel matrix formed, inside a hollow core Photonic Crystal Fibre (HC-PCF). We show both the sensitivity of fluorescence detection inside the HC-PCF using a supercontinuum light source and of the variation of the luminescence intensity with the concentration DNA probe within the hydrogel. The 3D hydrogel matrix is a network of polymer chains, which is expected to provide highly sensitive detection and selection of bio-molecules, in comparison with 2D coverage. The biocompatibility of hydrogel in the HC-PCF suggests numerous applications associated with immobilised DNA probe detection for point-of-care or remote systems.

Citation Download Citation

Monika Rutowska, Jiadi Lu, Fatima C. Garcia-Gunning, and Andrew D. Ellis "DNA probe detection within 3D hydrogel matrix in a hollow core photonic crystal fibre", Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77536I (17 May 2011); https://doi.org/10.1117/12.886070

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