Optical trapping enabled parallel delivery of biological stimuli with high spatial and temporal resolution

Daniel R Burnham; Thomas Schneider; Daniel T. Chiu

doi:10.1117/12.862519

27 August 2010 Optical trapping enabled parallel delivery of biological stimuli with high spatial and temporal resolution

Daniel R Burnham, Thomas Schneider, Daniel T. Chiu

Author Affiliations +

Proceedings Volume 7762, Optical Trapping and Optical Micromanipulation VII; 77621T (2010) https://doi.org/10.1117/12.862519
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

We have developed a method that employs nanocapsules, optical trapping, and single-pulse laser photolysis for delivering bioactive molecules to cells with both high spatial and temporal resolutions. This method is particularly suitable for a cell-culture setting, in which a single nanocapsule can be optically trapped and positioned at a pre-defined location next to the cell, followed by single-pulse laser photolysis to release the contents of the nanocapsule onto the cell. To parallelize this method such that a large array of nanocapsules can be manipulated, positioned, and photolyzed simultaneously, we have turned to the use of spatial light modulators and holographic beam shaping techniques. This paper outlines the progress we have made so far and details the issues we had to address in order to achieve efficient parallel optical manipulations of nanocapsules and particles.

Citation Download Citation

Daniel R Burnham, Thomas Schneider, and Daniel T. Chiu "Optical trapping enabled parallel delivery of biological stimuli with high spatial and temporal resolution", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621T (27 August 2010); https://doi.org/10.1117/12.862519

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