Non-invasive monitoring for living cell culture with lensless Fourier transform digital holography microscopy

Yunxin Wang; Dayong Wang; Jie Zhao; Yan Li; Puhui Meng; Yuhong Wan; Zhuqing Jiang

doi:10.1117/12.860164

2 August 2010 Non-invasive monitoring for living cell culture with lensless Fourier transform digital holography microscopy

Yunxin Wang, Dayong Wang, Jie Zhao, Yan Li, Puhui Meng, Yuhong Wan, Zhuqing Jiang

Author Affiliations +

Proceedings Volume 7791, Interferometry XV: Applications; 77910E (2010) https://doi.org/10.1117/12.860164
Event: SPIE Optical Engineering + Applications, 2010, San Diego, California, United States

Abstract

The number of cells is commonly employed to describe the cell viability and the status of cell culture in a certain extent. An automatic and non-invasive detecting method for the status analysis of cell culture is developed based on digital holography microscopy (DHM) technology. Digital holographic imaging can retrieve quantitative information of object wavefront by the numerical reconstruction from a single digital hologram recorded by a detector such as CCD or CMOS camera, which is especially suitable for the morphology detection of the transparent or semi-transparent cells. In this contribution, the lensless Fourier transform (LFT) based holography configuration is designed for cell imaging without prestaining, and the amplitude and phase of living cells can be reconstructed by digital reconstruction and phase unwrapped algorithms. Then the image filtering and segmentation are combined for the automatic evaluation of the level of confluency. In imaging experiments, the culture status of the cervical cancer cell TZMbl is detected, and the results demonstrate that digital holography microscopy provides a feasible non-invasive method for monitoring the living cell culture.

Citation Download Citation

Yunxin Wang, Dayong Wang, Jie Zhao, Yan Li, Puhui Meng, Yuhong Wan, and Zhuqing Jiang "Non-invasive monitoring for living cell culture with lensless Fourier transform digital holography microscopy", Proc. SPIE 7791, Interferometry XV: Applications, 77910E (2 August 2010); https://doi.org/10.1117/12.860164

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