Unzipping force analysis of protein association (UFAPA): a novel technique to probe protein-DNA interactions

Michelle D Wang; Steven J. Koch; Alla Shundrovsky; Benjamin C. Jantzen

doi:10.1117/12.500332

30 April 2003 Unzipping force analysis of protein association (UFAPA): a novel technique to probe protein-DNA interactions

Michelle D Wang, Steven J. Koch, Alla Shundrovsky, Benjamin C. Jantzen

Author Affiliations +

Proceedings Volume 5110, Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems; (2003) https://doi.org/10.1117/12.500332
Event: SPIE's First International Symposium on Fluctuations and Noise, 2003, Santa Fe, New Mexico, United States

Abstract

We present Unzipping Force Analysis of Protein Association (UFAPA) as a novel and versatile method for detection of the position and dynamic nature of protein-DNA interactions. A single DNA double helix was unzipped in the presence of DNA-binding proteins using a feedback-enhanced optical trap. When the unzipping fork in a DNA reached a bound protein molecule, we observed a dramatic increase in the tension in the DNA, followed by a sudden tension reduction. Analysis of the unzipping force throughout an unbinding "event" revealed information about the spatial location and dynamic nature of the protein-DNA complex.

Citation Download Citation

Michelle D Wang, Steven J. Koch, Alla Shundrovsky, and Benjamin C. Jantzen "Unzipping force analysis of protein association (UFAPA): a novel technique to probe protein-DNA interactions", Proc. SPIE 5110, Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems, (30 April 2003); https://doi.org/10.1117/12.500332

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available