Accuracy of a simple diffusion model of fluorescence for estimating fluorophore concentration

Kevin R. Diamond; Michael S Patterson; Thomas J Farrell

doi:10.1117/12.499820

15 October 2003 Accuracy of a simple diffusion theory model of fluorescence for estimating fluorophore concentration

Kevin R. Diamond, Michael S Patterson, Thomas J Farrell

Proceedings Volume 5138, Photon Migration and Diffuse-Light Imaging; (2003) https://doi.org/10.1117/12.499820
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Monte Carlo simulations and experiments in tissue-simulating phantoms were performed to study the accuracy of fluorophore concentrations recovered using a diffusion theory model of fluorescence. Fluorophore concentration was measured using three fluorophores with an overall root-mean-square accuracy of 13.7%. These experiments were compared with concentration measurements by diffuse reflectance spectroscopy, which were less accurate for all three fluorophores. Monte Carlo simulations were also used to examine the ability of the model to recover concentration from layered fluorophore distributions.

Citation Download Citation

Kevin R. Diamond, Michael S Patterson, and Thomas J Farrell "Accuracy of a simple diffusion theory model of fluorescence for estimating fluorophore concentration", Proc. SPIE 5138, Photon Migration and Diffuse-Light Imaging, (15 October 2003); https://doi.org/10.1117/12.499820

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