Raman and surface enhanced Raman spectroscopy of amino acids and peptide

Xiaojuan Yuan; Huaimin Gu; Jiwei Wu; Jian Kang; Xiao Dong

doi:10.1117/12.843500

28 October 2009 Raman and surface enhanced Raman spectroscopy of amino acids and peptide

Xiaojuan Yuan, Huaimin Gu, Jiwei Wu, Jian Kang, Xiao Dong

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75191D (2009) https://doi.org/10.1117/12.843500
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

Surface enhanced Raman scattering (SERS) is potentially tool in the characterization of biomolecules such as amino acids, complicated peptides and proteins, and even tissues or living cells. Amino acids and short peptides contain different functional groups. Therefore, they are suitable for the investigations of the competitive-interactions of these functional groups with colloidal silver surfaces. In this paper, Normal Raman and SERS of amino acids Leucine and Isoleucine and short peptide Leu-Leu were measured on the silver colloidal substrate. Raman shifts that stem from different vibrational mode in the molecular inner structure, and the variations of SERS of the samples were analyzed in this study. The results show that different connection of one methyl to the main chains of the isomer amino acids resulted in different vibration modes in the Normal Raman spectra of Leucine and Isoleucine. In the SERS spectra of the isomer amino acids, all frequency shifts are expressed more differently than those in Normal Raman spectra of solid state. Orientation of this isomer amino acids, as well as specific-competitive interactions of their functional groups with the colloidal silver surface, were speculated by detailed spectral analysis of the obtained SERS spectra. In addition, the dipeptide Leu-Leu, as the corresponding homodipeptide of Leucine, was also measured adsorbed on the colloidal silver surface. The SERS spectrum of Leu-Leu is different from its corresponding amino acid Leucine but both of them are adsorbed on the silver surface through the carboxylate moiety.

Citation Download Citation

Xiaojuan Yuan, Huaimin Gu, Jiwei Wu, Jian Kang, and Xiao Dong "Raman and surface enhanced Raman spectroscopy of amino acids and peptide", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75191D (28 October 2009); https://doi.org/10.1117/12.843500

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KEYWORDS

Surface enhanced Raman spectroscopy

Silver

Raman spectroscopy

Natural surfaces

Proteins

Raman scattering

Solid state physics

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