Real-time mapping of the corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy

Rudolf F. Guthoff; Andrey Zhivov; Oliver Stachs

doi:10.1117/12.842476

2 March 2010 Real-time mapping of the corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy

Rudolf F. Guthoff, Andrey Zhivov, Oliver Stachs

Proceedings Volume 7550, Ophthalmic Technologies XX; 75501D (2010) https://doi.org/10.1117/12.842476
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The aim of the study was to produce two-dimensional reconstruction maps of the living corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy in real time. CLSM source data (frame rate 30Hz, 384x384 pixel) were used to create large-scale maps of the scanned area by selecting the Automatic Real Time (ART) composite mode. The mapping algorithm is based on an affine transformation. Microscopy of the sub-basal nerve plexus was performed on normal and LASIK eyes as well as on rabbit eyes. Real-time mapping of the sub-basal nerve plexus was performed in large-scale up to a size of 3.2mm x 3.2mm. The developed method enables a real-time in vivo mapping of the sub-basal nerve plexus which is stringently necessary for statistically firmed conclusions about morphometric plexus alterations.

Citation Download Citation

Rudolf F. Guthoff, Andrey Zhivov, and Oliver Stachs "Real-time mapping of the corneal sub-basal nerve plexus by in vivo laser scanning confocal microscopy", Proc. SPIE 7550, Ophthalmic Technologies XX, 75501D (2 March 2010); https://doi.org/10.1117/12.842476

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