Small animal ocular biometry using optical coherence tomography

Marco Ruggeri; Omer Kocaoglu; Stephen Uhlhorn; David Borja; Raksha Urs; Tsung-Han Chou; Vittorio Porciatti; Jean-Marie Parel; Fabrice Manns

doi:10.1117/12.846937

2 March 2010 Small animal ocular biometry using optical coherence tomography

Marco Ruggeri, Omer Kocaoglu, Stephen Uhlhorn, David Borja, Raksha Urs, Tsung-Han Chou, Vittorio Porciatti, Jean-Marie Parel, Fabrice Manns

Author Affiliations +

Proceedings Volume 7550, Ophthalmic Technologies XX; 755016 (2010) https://doi.org/10.1117/12.846937
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A custom-built OCT system was used to obtain images of the whole mouse eye. We developed a semi-automated segmentation method to detect the boundaries of the anterior and posterior corneal, lens and retinal surfaces as well as the anterior surface of the iris. The radii of curvature of the surfaces were calculated using a conic section fit of each boundary. Image distortions due to refraction of the OCT beam at the successive boundaries were corrected using a ray-tracing algorithm. Corrected ocular distances, radii of curvature of the cornea and lens surfaces, and anterior chamber angle were obtained on 3 C57BL/6J mice. In vivo imaging of the whole eye, segmentation, conic function fits and correction were successful in all three animals. The posterior lens surface of one mouse could not be fit accurately with a conic section. Biometric parameters of C57BL/6J mice compared well with previous published data obtained from histological sections. The study demonstrates the feasibility of quantitative in vivo biometry of mouse models.

Citation Download Citation

Marco Ruggeri, Omer Kocaoglu, Stephen Uhlhorn, David Borja, Raksha Urs, Tsung-Han Chou, Vittorio Porciatti, Jean-Marie Parel, and Fabrice Manns "Small animal ocular biometry using optical coherence tomography", Proc. SPIE 7550, Ophthalmic Technologies XX, 755016 (2 March 2010); https://doi.org/10.1117/12.846937

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