Lung alveolar wall disruption in three-dimensional space identified using second-harmonic generation and multiphoton excitation fluorescence

Thomas Abraham; James Hogg M.D.

doi:10.1117/12.842321

22 February 2010 Lung alveolar wall disruption in three-dimensional space identified using second-harmonic generation and multiphoton excitation fluorescence

Thomas Abraham, James Hogg M.D.

Author Affiliations +

Proceedings Volume 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII; 75550X (2010) https://doi.org/10.1117/12.842321
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Second harmonic generation and multiphoton excited fluorescence microscopy methods were used to examine structural remodeling of the extracellular matrix in human lung alveolar walls undergoing emphysematous destruction. Fresh lung samples removed from a patient undergoing lung transplantation for very severe chronic obstructive pulmonary disease were compared to similar samples from an unused donor lung that served as a control. The generated spatially resolved 3D images show the spatial distribution of collagen, elastin and other endogenously fluorescent tissue components such as macrophages. In the case of control lung tissue, we found well ordered alveolar walls with composite type structure made up of collagen matrix and relatively fine elastic fibers. In contrast, lung tissue undergoing emphysematous destruction was highly disorganized with increased alveolar wall thickness compared to control lung tissue.

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Thomas Abraham and James Hogg M.D. "Lung alveolar wall disruption in three-dimensional space identified using second-harmonic generation and multiphoton excitation fluorescence", Proc. SPIE 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII, 75550X (22 February 2010); https://doi.org/10.1117/12.842321

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