Photoacoustic micro-imaging of focused ultrasound induced blood-brain-barrier opening in a rat model

Po-Hsun Wang; Po-Hung Hsu; Hao-Li Liu; Churng-Ren Chris Wang; Meng-Lin Li

doi:10.1117/12.841811

23 February 2010 Photoacoustic micro-imaging of focused ultrasound induced blood-brain-barrier opening in a rat model

Po-Hsun Wang, Po-Hung Hsu, Hao-Li Liu, Churng-Ren Chris Wang, Meng-Lin Li

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75642U (2010) https://doi.org/10.1117/12.841811
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Blood brain barrier (BBB) prevents most of the drug from transmitting into the brain tissue and decreases the treatment performance for brain disease. One of the methods to overcome the difficulty of drug delivery is to locally increase the permeability of BBB with high-intensity focused ultrasound. In this study, we have investigated the feasibility of photoacoustic microscopy of focused-ultrasound induced BBB opening in a rat model in vivo with gold nanorods (AuNRs) as a contrast agent. This study takes advantage of the strong near-infrared absorption of AuNRs and their extravasation tendency from BBB opening foci due to their nano-scale size. Before the experiments, craniotomy was performed on rats to provide a path for focused ultrasound beam. Localized BBB opening at the depth of about 3 mm from left cortex of rat brains was achieved by delivering 1.5 MHz focused ultrasound energy into brain tissue in the presence of microbubbles. PEGylated AuNRs with a peak optical absorption at ~800 nm were then intravenously administered. Pre-scan prior to BBB disruption and AuNR injection was taken to mark the signal background. After injection, the distribution of AuNRs in rat brains was monitored up to 2 hours. Experimental results show that imaging AuNRs reveals BBB disruption area in left brains while there are no changes observed in the right brains. From our results, photoacoustic imaging plus AuNRs shows the promise as a novel monitoring strategy in identifying the location and variation of focused-ultrasound BBB-opening in a rat model.

Citation Download Citation

Po-Hsun Wang, Po-Hung Hsu, Hao-Li Liu, Churng-Ren Chris Wang, and Meng-Lin Li "Photoacoustic micro-imaging of focused ultrasound induced blood-brain-barrier opening in a rat model", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642U (23 February 2010); https://doi.org/10.1117/12.841811

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Blood brain barrier

Brain

Ultrasonography

Absorption

Photoacoustic spectroscopy

In vivo imaging

Neuroimaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years