In vivo label-free photoacoustic microscopy of the anterior segment of the mouse eye

Bin Rao; Song Hu; Li Li; Konstantin Maslov; Lihong V. Wang

doi:10.1117/12.843052

23 February 2010 In vivo label-free photoacoustic microscopy of the anterior segment of the mouse eye

Bin Rao, Song Hu, Li Li, Konstantin Maslov, Lihong V. Wang

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 75643E (2010) https://doi.org/10.1117/12.843052
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Both iris fluorescein angiography (IFA) and indocyanine green angiography (ICGA) provide ophthalmologists imaging tools in studying the microvasculature structure and hemodynamics of the anterior segment of the eye in normal and diseased status. However, a non-invasive, endogenous imaging modality is preferable for the monitoring of hemodynamics of the iris microvasculature. We investigated the in vivo, label-free ocular anterior segment imaging with photo-acoustic microscopy (PAM) in mouse eyes. We demonstrated the unique advantage of endogenous contrast that is not available in both IFA and ICGA. The laser radiation was maintained within the ANSI laser safety limit. The in vivo, label-free nature of our imaging technology has the potential for ophthalmic applications.

Citation Download Citation

Bin Rao, Song Hu, Li Li, Konstantin Maslov, and Lihong V. Wang "In vivo label-free photoacoustic microscopy of the anterior segment of the mouse eye", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643E (23 February 2010); https://doi.org/10.1117/12.843052

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available