Enhanced detection of circulating melanoma cells using gold nanoparticles as photoacoustic contrasting agents

Devin R. McCormack; Kiran Bhattacharyya; Raghuraman Kannan; Kattesh Katti; John A. Viator

doi:10.1117/12.841474

23 February 2010 Enhanced detection of circulating melanoma cells using gold nanoparticles as photoacoustic contrasting agents

Devin R. McCormack, Kiran Bhattacharyya, Raghuraman Kannan, Kattesh Katti, John A. Viator

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 756413 (2010) https://doi.org/10.1117/12.841474
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Nanotechnology and the various properties of gold nanoparticles (AuNPs) are quickly changing the field of cancer detection and treatment. Photoacoustic detection methods show an increase in sensitivity using gold nanoparticle antibody conjugation, which selectively targets melanoma cancer cells. Instead of targeting melanoma tumors, we tag single cells, analogous to circulating metastatic melanoma cells. Using an in vitro, stationary cell system and planar samples, we demonstrate an average of 24% improved optical detectability of melanoma cells tagged with AuNPs over unprocessed melanoma cells. Tagged cells showed a raised plateau of absorbance from 470nm to 550nm. Untagged cells showed a general decline in absorption as wavelength increased. The results of our study have the potential to not only better develop photoacoustic detection of melanoma, but also extend the viability and use of photoacoustics into detection of otherwise unpigmented cancers.

Citation Download Citation

Devin R. McCormack, Kiran Bhattacharyya, Raghuraman Kannan, Kattesh Katti, and John A. Viator "Enhanced detection of circulating melanoma cells using gold nanoparticles as photoacoustic contrasting agents", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756413 (23 February 2010); https://doi.org/10.1117/12.841474

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available