MICAO: first universal all-in-the-box adaptive optics plug in accessory for standard high resolution microscopy

J. Andilla; X. Levecq

doi:10.1117/12.845247

24 February 2010 MICAO: first universal all-in-the-box adaptive optics plug in accessory for standard high resolution microscopy

J. Andilla, X. Levecq

Author Affiliations +

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75680U (2010) https://doi.org/10.1117/12.845247
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We present MICAO, an accessory which implements adaptive optics for microscopy. The device is designed to be plugged into the camera port of an inverted microscope to correct for the wavefront aberrations introduced by the system. It is compatible with most of the microscopes and can be easily installed in an inverted microscope imaging system. The design of the system allows working with almost all of the biological objectives. We also present the sensorless correction algorithm used to improve the image quality. Finally, we demonstrate the capability of the system to correct the aberrations present into a sample designed specially.

Citation Download Citation

J. Andilla and X. Levecq "MICAO: first universal all-in-the-box adaptive optics plug in accessory for standard high resolution microscopy", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680U (24 February 2010); https://doi.org/10.1117/12.845247

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