Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion

William M. Ash III; David Clark; Chun-Min Lo; Myung K. Kim

doi:10.1117/12.842470

11 February 2010 Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion

William M. Ash III, David Clark, Chun-Min Lo, Myung K. Kim

Author Affiliations +

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 756803 (2010) https://doi.org/10.1117/12.842470
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A new form of near-field microscopy is presented using digital holography for quantitative phase imagery and characterization of cell-substrate interfaces. This imaging technique, termed total internal reflection holographic microscopy (TIRHM), utilizes an evanescent wave phase shift from the presence of cellular organisms, membranes, adhesions, and tissue structures on a prism face in order to modulate an object beam wavefront in a digital holographic microscope. Quantitative phase images of live cellular specimens are presented.

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William M. Ash III, David Clark, Chun-Min Lo, and Myung K. Kim "Total internal reflection holographic microscopy for quantitative phase characterization of cellular adhesion", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756803 (11 February 2010); https://doi.org/10.1117/12.842470

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