Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy

Wei-Liang Chen; Tsung-Hsian Li; Ping-Jung Su; Chen-Kuan Chou; Peter Tramyeon Fwu; Sung-Jan Lin; Daekeun Kim; Peter T. C. So; Chen-Yuan Dong

doi:10.1117/12.843015

26 February 2010 Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy

Wei-Liang Chen, Tsung-Hsian Li, Ping-Jung Su, Chen-Kuan Chou, Peter Tramyeon Fwu, Sung-Jan Lin, Daekeun Kim, Peter T. C. So, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75691P (2010) https://doi.org/10.1117/12.843015
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Second harmonic generation (SHG) microscopy has become an important tool for minimally invasive biomedical imaging. However, differentiation of different second harmonic generating species is mainly provided by morphological features. Using excitation polarization-resolved SHG microscopy we determined the ratios of the second-order susceptibility tensor elements at single pixel resolution. Mapping the resultant ratios for each pixel onto an image provides additional contrast for the differentiation of different sources of SHG. We demonstrate this technique by imaging collagen-muscle junction of chicken wing.

Citation Download Citation

Wei-Liang Chen, Tsung-Hsian Li, Ping-Jung Su, Chen-Kuan Chou, Peter Tramyeon Fwu, Sung-Jan Lin, Daekeun Kim, Peter T. C. So, and Chen-Yuan Dong "Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691P (26 February 2010); https://doi.org/10.1117/12.843015

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available