In vivo multiphoton imaging of obstructive cholestasis in mice

Feng-Chieh Li; Yu Yang Lee; Ling-Ling Chiou; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/12.843096

26 February 2010 In vivo multiphoton imaging of obstructive cholestasis in mice

Feng-Chieh Li, Yu Yang Lee, Ling-Ling Chiou, Hsuan-Shu Lee, Chen-Yuan Dong

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692C (2010) https://doi.org/10.1117/12.843096
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Combining multiphoton microscopy with a newly designed hepatic imaging window, we acquired in vivo images of mice obstructive cholestasis. We observed that in mice with bile duct ligation, bile canaliculi failed to appear during the whole observation period over 100 minutes following carboxyfluorescein diacetate injection, whereas the fluorescence was retained much longer within sinusoids. Furthermore, the fluorescence intensities in sinusoids were persistently higher than in hepatocytes during the course.

Citation Download Citation

Feng-Chieh Li, Yu Yang Lee, Ling-Ling Chiou, Hsuan-Shu Lee, and Chen-Yuan Dong "In vivo multiphoton imaging of obstructive cholestasis in mice", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692C (26 February 2010); https://doi.org/10.1117/12.843096

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KEYWORDS

Luminescence

Liver

In vivo imaging

Multiphoton microscopy

Veins

Blood

Medicine

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