Digital holographic microscopy at fundamental and second harmonic wavelengths

Etienne Shaffer; Christian Depeursinge

doi:10.1117/12.831494

1 July 2009 Digital holographic microscopy at fundamental and second harmonic wavelengths

Proceedings Volume 7367, Advanced Microscopy Techniques; 736716 (2009) https://doi.org/10.1117/12.831494
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering of the complex diffraction wavefields from which it is possible to extract both amplitude-contrast and quantitative phase images. We believe that application of digital holography to non-linear optical fields might ultimately be the key to phase-related functional imaging. In a first approach, we report here on second harmonic generation digital holographic microscopy, and present its application to (1) discrimination of nanoparticles of different nature - here polystyrene microspheres and barium titanate (BaTiO3) nanoparticles - and to (2) 3D-mapping of a distribution of BaTiO₃ nanoparticles.

Citation Download Citation

Etienne Shaffer and Christian Depeursinge "Digital holographic microscopy at fundamental and second harmonic wavelengths", Proc. SPIE 7367, Advanced Microscopy Techniques, 736716 (1 July 2009); https://doi.org/10.1117/12.831494

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