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Proceedings Article

Application of color digital holographic microscopy for analysis of stained tissue sections

[+] Author Affiliations
Xiaoli Mo

Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany) and Beijing Institute of Technology (China)

Björn Kemper, Patrik Langehanenberg, Angelika Vollmer, Gert von Bally

Ctr. for Biomedical Optics and Photonics, Univ. of Muenster (Germany)

Jinghui Xie

Beijing Institute of Technology (China)

Proc. SPIE 7367, Advanced Microscopy Techniques, 736718 (July 01, 2009); doi:10.1117/12.831476
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From Conference Volume 7367

  • Advanced Microscopy Techniques
  • Paul J. Campagnola; Ernst H. K. Stelzer; Gert von Bally
  • Munich, Germany | June 14, 2009

abstract

The analysis of stained tissue sections represents an important tool in medical diagnostics. Color digital holographic microscopy offers subsequent multi-focus true color imaging with simultaneous quantitative phase contrast analysis. Investigations on digital recording and numerical reconstructions of color digital holographic images have been performed by applying a transmission microscope experimental setup in holographic off-axis geometry. Three monochromatic digital holograms with different wavelengths in red, green and blue spectral range are recorded. After digital holographic refocusing and compensation for aberrations of the microscope imaging system by digital image correlation the numerically reconstructed amplitude distributions are combined into color images. The applicability of the method is demonstrated by results obtained from stained intestine tissue sections.

© (2009) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Xiaoli Mo ; Björn Kemper ; Patrik Langehanenberg ; Angelika Vollmer ; Jinghui Xie, et al.
"Application of color digital holographic microscopy for analysis of stained tissue sections", Proc. SPIE 7367, Advanced Microscopy Techniques, 736718 (July 01, 2009); doi:10.1117/12.831476; http://dx.doi.org/10.1117/12.831476


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