A multimodal multiphoton microscope for biological imaging

Rabah Mouras; Andrew Downes; Grigore Rischitor; Meropi Mari; Alistair Elfick

doi:10.1117/12.844236

26 February 2010 A multimodal multiphoton microscope for biological imaging

Rabah Mouras, Andrew Downes, Grigore Rischitor, Meropi Mari, Alistair Elfick

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756933 (2010) https://doi.org/10.1117/12.844236
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We report on the construction of a highly flexible system for advanced biological imaging, where all the following imaging techniques are integrated into the same microscope: Coherent anti-Stokes Raman scattering (CARS), two photon excitation fluorescence (TPEF), second harmonic generation (SGH), sum frequency generation (SFG), fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC). The system employs a Nd:YVO₄ laser as pump (7 ps, 1064 nm), and two tunable OPOs (6 ps, 700 - 1000 nm). Our microscope comprises a heater stage and perfusion cell for imaging of live cells, and features an atomic force microscope (AFM) which enables optical imaging at 10 nm resolution. Multimodal imaging of breast cancer cells and tissue will be demonstrated as well as imaging of anticancer drugs in living cells.

Citation Download Citation

Rabah Mouras, Andrew Downes, Grigore Rischitor, Meropi Mari, and Alistair Elfick "A multimodal multiphoton microscope for biological imaging", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756933 (26 February 2010); https://doi.org/10.1117/12.844236

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