Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging

Pierre Bon; Benoit Wattellier; Serge Monneret; Didier Marguet

doi:10.1117/12.844930

24 February 2010 Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging

Pierre Bon, Benoit Wattellier, Serge Monneret, Didier Marguet

Author Affiliations +

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700E (2010) https://doi.org/10.1117/12.844930
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. We propose here to study long-time duration imaging on different type of adherent cells: green monkey kidney COS7 cells, human breast epithelial MCF10A cells, and human breast cancer derived from MDA-231 cells. This study permits a enhanced visualization of the whole cell life at different levels of confluence. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details and thresholding.

Citation Download Citation

Pierre Bon, Benoit Wattellier, Serge Monneret, and Didier Marguet "Quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700E (24 February 2010); https://doi.org/10.1117/12.844930

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