dSTORM: real-time subdiffraction-resolution fluorescence imaging with organic fluorophores

Mark Schüttpelz; Steve Wolter; Sebastian van de Linde; Mike Heilemann; Markus Sauer

doi:10.1117/12.848105

25 February 2010 dSTORM: real-time subdiffraction-resolution fluorescence imaging with organic fluorophores

Mark Schüttpelz, Steve Wolter, Sebastian van de Linde, Mike Heilemann, Markus Sauer

Proceedings Volume 7571, Single Molecule Spectroscopy and Imaging III; 75710V (2010) https://doi.org/10.1117/12.848105
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In the recent past, a variety of methods have been developed to circumvent the diffraction barrier of light which restricts optical resolution to about 200 nm in the image plane. Single-molecule based photoswitching microscopy such as direct stochastic optical reconstruction microscopy (dSTORM) has been successfully implemented for subdiffraction-resolution fluorescence imaging. The major drawback of this technique has been that the reconstruction of subdiffraction-resolution images requires substantially more time than the actual experiment and prevented real-time imaging. Here we present a new computational algorithm enabling subdiffraction-resolution fast imaging of cellular structures with ~20 nm optical resolution in less than 10 seconds.

Citation Download Citation

Mark Schüttpelz, Steve Wolter, Sebastian van de Linde, Mike Heilemann, and Markus Sauer "dSTORM: real-time subdiffraction-resolution fluorescence imaging with organic fluorophores", Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 75710V (25 February 2010); https://doi.org/10.1117/12.848105

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available