Exploiting of optical transforms for bacteria evaluation in vitro

Igor Buzalewicz; Katarzyna Wysocka; Halina Podbielska

doi:10.1117/12.831777

9 July 2009 Exploiting of optical transforms for bacteria evaluation in vitro

Igor Buzalewicz, Katarzyna Wysocka, Halina Podbielska

Proceedings Volume 7371, Novel Optical Instrumentation for Biomedical Applications IV; 73711H (2009) https://doi.org/10.1117/12.831777
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

A novel method for evaluation of bacteria colonies concentration based on optical spectra examination, is proposed. The influence of bacteria colonies number on Fourier spectrum properties is considered in term of scalar diffraction theory and corresponding theoretical model is presented. Computational simulations are performed to confirm the theoretical predictions. Additionally, optical Mellin transform is used to omit the dependence of Fourier spectrum pattern on bacteria colonies size fluctuations and to provide a scale-invariant analysis. Presented results have shown high potential of the proposed approach for comparative study of bacteria colonies grown on solid medium in vitro.

Citation Download Citation

Igor Buzalewicz, Katarzyna Wysocka, and Halina Podbielska "Exploiting of optical transforms for bacteria evaluation in vitro", Proc. SPIE 7371, Novel Optical Instrumentation for Biomedical Applications IV, 73711H (9 July 2009); https://doi.org/10.1117/12.831777

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available