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Proceedings Article

Exploiting of optical transforms for bacteria evaluation in vitro

[+] Author Affiliations
Igor Buzalewicz, Katarzyna Wysocka, Halina Podbielska

Bio-Optics Group, Institute of Biomedical Engineering & Instrumentation, Wroclaw Univ. of Technology (Poland)

Proc. SPIE 7371, Novel Optical Instrumentation for Biomedical Applications IV, 73711H (July 09, 2009); doi:10.1117/12.831777
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From Conference Volume 7371

  • Novel Optical Instrumentation for Biomedical Applications IV
  • Christian D. Depeursinge; I. Alex Vitkin
  • Munich, Germany | June 14, 2009

abstract

A novel method for evaluation of bacteria colonies concentration based on optical spectra examination, is proposed. The influence of bacteria colonies number on Fourier spectrum properties is considered in term of scalar diffraction theory and corresponding theoretical model is presented. Computational simulations are performed to confirm the theoretical predictions. Additionally, optical Mellin transform is used to omit the dependence of Fourier spectrum pattern on bacteria colonies size fluctuations and to provide a scale-invariant analysis. Presented results have shown high potential of the proposed approach for comparative study of bacteria colonies grown on solid medium in vitro.

© (2009) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Igor Buzalewicz ; Katarzyna Wysocka and Halina Podbielska
"Exploiting of optical transforms for bacteria evaluation in vitro", Proc. SPIE 7371, Novel Optical Instrumentation for Biomedical Applications IV, 73711H (July 09, 2009); doi:10.1117/12.831777; http://dx.doi.org/10.1117/12.831777


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