A fiber-coupled microfluidic cytometer for the obtaining of nanostructural mitochondria information in single cells

Xuantao Su; Wojciech Rozmus; Ying Y. Tsui

doi:10.1117/12.841179

26 February 2010 A fiber-coupled microfluidic cytometer for the obtaining of nanostructural mitochondria information in single cells

Xuantao Su, Wojciech Rozmus, Ying Y. Tsui

Author Affiliations +

Proceedings Volume 7573, Biomedical Applications of Light Scattering IV; 75730K (2010) https://doi.org/10.1117/12.841179
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

A fiber-coupled microfluidic cytometer has been recently developed in our group for the obtaining of 2D light scatter patterns from single biological cells. The obtained scatter patterns may be used for label-free characterization of biological cells. Understanding of these 2D scatter patterns can be better achieved by comparing the experimental results with those obtained by theoretical simulations, such as the finite-difference time-domain (FDTD) simulation of light scattering from biological cells. In this paper, we provide detailed study for applying the FDTD method in our fibercoupled microfluidic cytometer. The FDTD simulation results agree reasonably well with the experimentally obtained THP-1 cell 2D scatter patterns. Methods for scatter pattern analysis are under development in our group for new light scattering parameters that may potentially be used in clinics.

Citation Download Citation

Xuantao Su, Wojciech Rozmus, and Ying Y. Tsui "A fiber-coupled microfluidic cytometer for the obtaining of nanostructural mitochondria information in single cells", Proc. SPIE 7573, Biomedical Applications of Light Scattering IV, 75730K (26 February 2010); https://doi.org/10.1117/12.841179

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