Development of an integrated microsystem for the multiplexed detection of breast cancer markers in serum using electrochemical immunosensors

Alex Fragoso; Noemi Laboria; Mary Luz Botero; Diego Bejarano; Daniel Latta; Thomas E. Hansen-Hagge; Wolfgang Kemmner; Ioanis Katakis; Claudia Gärtner; Klaus Drese; Ciara K. O'Sullivan

doi:10.1117/12.857311

23 February 2010 Development of an integrated microsystem for the multiplexed detection of breast cancer markers in serum using electrochemical immunosensors

Alex Fragoso, Noemi Laboria, Mary Luz Botero, Diego Bejarano, Daniel Latta, Thomas E. Hansen-Hagge, Wolfgang Kemmner, Ioanis Katakis, Claudia Gärtner, Klaus Drese, Ciara K. O'Sullivan

Author Affiliations +

Proceedings Volume 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII; 75930K (2010) https://doi.org/10.1117/12.857311
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

A microsystem integrating electrochemical biosensoric detection for the simultaneous multiplexed detection of protein markers of breast cancer is reported. The immobilization of antibodies against each of carcinoembryonic antigen (CEA), prostate specific antigen (PSA) and cancer antigen 15-3 (CA15-3) was achieved via crosslinking to a bipodal dithiol chemisorbed on gold electrodes. This bipodal dithiol had the double function of eliminating non-specific binding and optimal spacing of the anchor antibodies for maximum accessibility to the target proteins. Storage conditions were optimized, demonstrating a long-term stability of the reporter conjugates jointly stored within a single reservoir in the microsystem. The final system has been optimized in terms of incubation times, temperatures and simultaneous, multiplexed detection of the protein markers was achieved in less than 10 minutes with less than ng/mL detection limits. The microsystem has been validated using real patient serum samples and excellent correlation with ELISA results obtained.

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Alex Fragoso, Noemi Laboria, Mary Luz Botero, Diego Bejarano, Daniel Latta, Thomas E. Hansen-Hagge, Wolfgang Kemmner, Ioanis Katakis, Claudia Gärtner, Klaus Drese, and Ciara K. O'Sullivan "Development of an integrated microsystem for the multiplexed detection of breast cancer markers in serum using electrochemical immunosensors", Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 75930K (23 February 2010); https://doi.org/10.1117/12.857311

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