Probing of cancer cell apoptosis by SERS and LSCM

Jian Kang; Huaimin Gu

doi:10.1117/12.835118

31 August 2009 Probing of cancer cell apoptosis by SERS and LSCM

Jian Kang, Huaimin Gu

Proceedings Volume 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging; 73824H (2009) https://doi.org/10.1117/12.835118
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

Surface enhanced Raman spectroscopy (SERS) can provide information of internal structures and chemical components from different kinds of samples. Laser scanning confocal microscopy (LSCM) can show morphologic information of samples by high-resolution optical images with different focal planes. In this paper, the dynamic variation of cancer cells (HELA cells) in the apoptosis was first studied by combining SERS and LSCM. After gold nanoparticles (GNPS) uptake, HELA cells were divided into two groups, and were respectively studied at six different time points of cell apoptosis period by SERS and LSCM. The LSCM images of HELA cells obtained at different time points were analyzed, and the morphology varieties of HELA cells apoptosis were obtained. It suggests that HELA cells apoptosis gradually in the apoptosis period until they died. In addition, Raman spectra of HELA cells measured at different time points were also compared. It shows that some Raman signal peaks shift, and FWHM of Raman peaks change too. The variation of internal structures and chemical constituents were analyzed according to the shifts and FWHM of the Raman peaks. The internal dynamic information and morphologic varieties from HELA cells apoptosis gained by combining SERS and LSCM will make us to understand cancer cell apoptosis throughly.

Citation Download Citation

Jian Kang and Huaimin Gu "Probing of cancer cell apoptosis by SERS and LSCM", Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 73824H (31 August 2009); https://doi.org/10.1117/12.835118

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