A method for three-dimensional quantitative observation of the microstructure of biological samples

Pengfei Wang; Dieyan Chen; Wanyun Ma; Hongxin Wu; Liang Ji; Jialin Sun; Danyu Lv; Lu Zhang; Ying Li; Ning Tian; Jinggao Zheng; Fengying Zhao

doi:10.1117/12.834098

31 August 2009 A method for three-dimensional quantitative observation of the microstructure of biological samples

Pengfei Wang, Dieyan Chen, Wanyun Ma, Hongxin Wu, Liang Ji, Jialin Sun, Danyu Lv, Lu Zhang, Ying Li, Ning Tian, Jinggao Zheng, Fengying Zhao

Author Affiliations +

Proceedings Volume 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging; 73824J (2009) https://doi.org/10.1117/12.834098
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

Contemporary biology has developed into the era of cell biology and molecular biology, and people try to study the mechanism of all kinds of biological phenomena at the microcosmic level now. Accurate description of the microstructure of biological samples is exigent need from many biomedical experiments. This paper introduces a method for 3-dimensional quantitative observation on the microstructure of vital biological samples based on two photon laser scanning microscopy (TPLSM). TPLSM is a novel kind of fluorescence microscopy, which has excellence in its low optical damage, high resolution, deep penetration depth and suitability for 3-dimensional (3D) imaging. Fluorescent stained samples were observed by TPLSM, and afterward the original shapes of them were obtained through 3D image reconstruction. The spatial distribution of all objects in samples as well as their volumes could be derived by image segmentation and mathematic calculation. Thus the 3-dimensionally and quantitatively depicted microstructure of the samples was finally derived. We applied this method to quantitative analysis of the spatial distribution of chromosomes in meiotic mouse oocytes at metaphase, and wonderful results came out last.

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Pengfei Wang, Dieyan Chen, Wanyun Ma, Hongxin Wu, Liang Ji, Jialin Sun, Danyu Lv, Lu Zhang, Ying Li, Ning Tian, Jinggao Zheng, and Fengying Zhao "A method for three-dimensional quantitative observation of the microstructure of biological samples", Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 73824J (31 August 2009); https://doi.org/10.1117/12.834098

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KEYWORDS

3D image processing

Image segmentation

Image processing

3D image reconstruction

Microscopy

Visualization

Luminescence

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