A PDMS sample pretreatment microdevice to enable downstream electrokinetic manipulations in bovine serum

Timothy J. Abram; David S. Clague

doi:10.1117/12.846448

17 February 2010 A PDMS sample pretreatment microdevice to enable downstream electrokinetic manipulations in bovine serum

Timothy J. Abram, David S. Clague

Author Affiliations +

Proceedings Volume 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII; 759318 (2010) https://doi.org/10.1117/12.846448
Event: SPIE MOEMS-MEMS, 2010, San Francisco, California, United States

Abstract

The notion of sample preconditioning, or pretreatment, as a micro-unit operation in a Lab on a Chip (LOC) system has yet to be realized in commercial practice. As is well known, Biomarker detection in complex, biological samples, such as blood, requires a series of pretreatment steps to enable detection of specific markers. On chip, such a process usually relies on "off-chip" sample pretreatment prior to "on-chip" analyte manipulations and detection. Presented in this paper is a PDMS, pretreatment chip based on the design of Oddy et al.1 with a view to enable a self-contained LOC platform. The chip was designed to directly manipulate the suspended species while adjusting fluid properties using buffer volumes less than 1 ml. Using previous literature related to capillary electrophoresis, a bench-scale pretreatment protocol was developed to tune specific fluidic parameters to an optimal range, namely pH, conductivity, and viscosity. A PDMS device was fabricated and used to combine a raw, bovine serum sample with specific buffer solutions. Off-chip electrodes were used to induce DC-electrokinetic micro-mixing of the target analyte in the mixing chamber, where a homogeneous analyte distribution was achieved in less than one second using an 800V DC pulse wave. Additionally, the desired solution viscosity and pH were achieved using less than 1 ml of buffer solution. Adjustment of sample conductivity, which is driven by sample fluid volume, remains an open area of research.

Citation Download Citation

Timothy J. Abram and David S. Clague "A PDMS sample pretreatment microdevice to enable downstream electrokinetic manipulations in bovine serum", Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 759318 (17 February 2010); https://doi.org/10.1117/12.846448

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