Reactive derivatives of gramicidin enable light- and ion-modulated ion channels

Michael X. Macrae; Steven Blake; Thomas Mayer; Michael Mayer; Jerry Yang

doi:10.1117/12.827686

21 August 2009 Reactive derivatives of gramicidin enable light- and ion-modulated ion channels

Michael X. Macrae, Steven Blake, Thomas Mayer, Michael Mayer, Jerry Yang

Proceedings Volume 7397, Biosensing II; 739709 (2009) https://doi.org/10.1117/12.827686
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

Detection of chemical processes on a single molecule scale is the ultimate goal of sensitive analytical assays. We have explored methods to detect chemical analytes in solution using synthetic derivatives of gramicidin A (gA). We exploited the functional properties of an ion channel-forming peptideg—gA—to report changes in the local environment near the opening of these semi-synthetic nanopores upon exposure to specific external stimuli. These peptide-based nanosensors detect reaction-induced changes in the chemical or physical properties of functional groups presented at the opening of the pore. This paper discusses the development of gA-based sensors for detecting external factors such as metal ions in solution or for detecting specific wavelengths of light. We propose that gA-based ion channel sensors offer tremendous potential for ultra sensitive functional detection since a single chemical modification of each individual sensing element can lead to readily detectable changes in channel conductance.

Citation Download Citation

Michael X. Macrae, Steven Blake, Thomas Mayer, Michael Mayer, and Jerry Yang "Reactive derivatives of gramicidin enable light- and ion-modulated ion channels", Proc. SPIE 7397, Biosensing II, 739709 (21 August 2009); https://doi.org/10.1117/12.827686

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