Depth of field extension in a low power microscope objective

Pantazis Mouroulis; Byron E. van Gorp; Holly A. Bender; Eric E. Bloemhof; Julia Nichols; Susanne Douglas

doi:10.1117/12.827393

21 August 2009 Depth of field extension in a low power microscope objective

Pantazis Mouroulis, Byron E. van Gorp, Holly A. Bender, Eric E. Bloemhof, Julia Nichols, Susanne Douglas

Proceedings Volume 7429, Novel Optical Systems Design and Optimization XII; 742904 (2009) https://doi.org/10.1117/12.827393
Event: SPIE Optical Engineering + Applications, 2009, San Diego, California, United States

Abstract

Three different techniques for extending the depth of field of a low-power (4x) microscope objective system are examined experimentally: wavefront coding with a cubic phase mask, amplitude modulation with a large central obscuration, and added spherical aberration. Their relative merits are discussed and demonstrated with sample images.

Citation Download Citation

Pantazis Mouroulis, Byron E. van Gorp, Holly A. Bender, Eric E. Bloemhof, Julia Nichols, and Susanne Douglas "Depth of field extension in a low power microscope objective", Proc. SPIE 7429, Novel Optical Systems Design and Optimization XII, 742904 (21 August 2009); https://doi.org/10.1117/12.827393

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