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We present the first experimental study of self-forming synthetic lipids, trademarked as QuSomesTM, using Raman
spectroscopy in the spectral range of 500 to 3100 cm-1. Raman spectra of these new artificial lipids composed of 1,2-
dimyristoyl-rac-glycerol-3-dodecaethylene glycol (GDM-12) and 1,2-dioleoyl-rac-glycerol-3-dodecaethylene glycol
(GDO-12) have been obtained in pure form and in aqueous suspensions with Phosphate Buffered Saline (PBS) by using
an inverted confocal laser-tweezers-Raman-microscopy system. This spectrometer works with an 80 mW diode-pumped
solid-state laser, operating at a wavelength of 785 nm in the TEM00 mode. The laser is used both for optical trapping and
Raman excitation. The two amphiphiles considered in this study, differ in their hydrophobic chain length and contain
similar units of hydrophilic polyethylene glycol (PEG) head groups. Such synthetic PEG coated lipids exist in liquid
form at room temperature and spontaneously form liposomes (nano type vesicles) upon hydration. In this work, we have
focused on the band assignments for the spectra of single QuSomesTM nano particles in pure form and in aqueous media
acquired by means of Raman spectroscopy. In particular, we have found that the most prominent peaks in the studied
spectral region are dominated by vibrational modes arising from C-C and C-H bonds. Furthermore, we have noticed
that some of the distinct peaks observed below 1800 cm-1 in pure sample are preserved in aqueous environment. These
retained intense bands are located at 1449, 1128, 1079, and 1065 cm-1. This effect might be due to the strong chain-chain
interactions, because the chains have to orient themselves and become tightly packed in the vesicles wall rather than
adopt random orientations in bulk. This technique has proven to be an excellent tool to establish the fingerprint region
revealing the molecular structure and conformation of QuSomesTM particles. The Raman spectroscopic data of these
novel lipids and its vesicles formed in suspensions confirm high stability and are therefore considered as potential
candidate for varieties of future applications including lipid based novel substances and drug delivery systems.
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