Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Human bladder cancer diagnosis using multiphoton microscopy

[+] Author Affiliations
Sushmita Mukherjee, James S. Wysock, Casey K. Ng, Mohammed Akhtar, Sven Perner, Ming-Ming Lee, Mark A. Rubin, Frederick R. Maxfield, Douglas S. Scherr

Weill Cornell Medical College (USA)

Watt W. Webb

Cornell Univ. (USA)

Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 716117 (February 23, 2009); doi:10.1117/12.808314
Text Size: A A A
From Conference Volume 7161

  • Photonic Therapeutics and Diagnostics V
  • Nikiforos Kollias; Bernard Choi; Haishan Zeng; Reza S. Malek; Brian J. Wong; Justus F. R. Ilgner; Kenton W. Gregory; Guillermo J. Tearney; Laura Marcu; Henry Hirschberg; Steen J. Madsen
  • San Jose, CA | January 24, 2009

abstract

At the time of diagnosis, approximately 75% of bladder cancers are non-muscle invasive. Appropriate diagnosis and surgical resection at this stage improves prognosis dramatically. However, these lesions, being small and/or flat, are often missed by conventional white-light cystoscopes. Furthermore, it is difficult to assess the surgical margin for negativity using conventional cystoscopes. Resultantly, the recurrence rates in patients with early bladder cancer are very high. This is currently addressed by repeat cystoscopies and biopsies, which can last throughout the life of a patient, increasing cost and patient morbidity. Multiphoton endoscopes offer a potential solution, allowing real time, noninvasive biopsies of the human bladder, as well as an up-close assessment of the resection margin. While miniaturization of the Multiphoton microscope into an endoscopic format is currently in progress, we present results here indicating that Multiphoton imaging (using a bench-top Multiphoton microscope) can indeed identify cancers in fresh, unfixed human bladder biopsies. Multiphoton images are acquired in two channels: (1) broadband autofluorescence from cells, and (2) second harmonic generation (SHG), mostly by tissue collagen. These images are then compared with gold standard hematoxylin/eosin (H&E) stained histopathology slides from the same specimen. Based on a "training set" and a very small "blinded set" of samples, we have found excellent correlation between the Multiphoton and histopathological diagnoses. A larger blinded analysis by two independent uropathologists is currently in progress. We expect that the conclusion of this phase will provide us with diagnostic accuracy estimates, as well as the degree of inter-observer heterogeneity.

© (2009) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Sushmita Mukherjee ; James S. Wysock ; Casey K. Ng ; Mohammed Akhtar ; Sven Perner, et al.
"Human bladder cancer diagnosis using multiphoton microscopy", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 716117 (February 23, 2009); doi:10.1117/12.808314; http://dx.doi.org/10.1117/12.808314


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.