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18 February 2009 Investigation on fibrous collagen modifications during corneal laser welding by second harmonic generation microscopy
Paolo Matteini, Fulvio Ratto, Francesca Rossi, Riccardo Cicchi, Chiara Stringari, Dimitrios Kapsokalyvas, Francesco S. Pavone, Roberto Pini
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Proceedings Volume 7163, Ophthalmic Technologies XIX; 71630Y (2009) https://doi.org/10.1117/12.808739
Event: SPIE BiOS, 2009, San Jose, California, United States
Abstract
The structural modifications of the collagen lattice induced in corneal stroma by laser welding were investigated with second-harmonic-generation (SHG) imaging. Corneal laser welding lies in the staining of the wound with a saturated solution of ICG followed by irradiation with a near-infrared-laser operated in continuous (CWLW) or pulsed (PWL) mode. After CWLW the lamellar arrangement was lost although a densely packing of collagen bundles increasingly disordered remained still clearly visible pointing out the lack of complete collagen denaturation. PLW produced welding spots which were characterized by a severe loss of SHG signal suggesting the occurrence of a complete collagen denaturation.
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Paolo Matteini, Fulvio Ratto, Francesca Rossi, Riccardo Cicchi, Chiara Stringari, Dimitrios Kapsokalyvas, Francesco S. Pavone, and Roberto Pini "Investigation on fibrous collagen modifications during corneal laser welding by second harmonic generation microscopy", Proc. SPIE 7163, Ophthalmic Technologies XIX, 71630Y (18 February 2009); https://doi.org/10.1117/12.808739
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KEYWORDS
Collagen
Second-harmonic generation
Laser welding
Cornea
Microscopy
Tissues
Signal attenuation
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