A novel concept for protein microarray: land-contrast BioCD

Xuefeng Wang; Ming Zhao; David D. Nolte

doi:10.1117/12.809358

23 February 2009 A novel concept for protein microarray: land-contrast BioCD

Xuefeng Wang, Ming Zhao, David D. Nolte

Proceedings Volume 7170, Design and Quality for Biomedical Technologies II; 717003 (2009) https://doi.org/10.1117/12.809358
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We present a new type of protein microarray called the Land-contrast (LC) BioCD in which imaging contrast is induced by a patterned substrate rather than by patterned protein. This is realized by etching spot patterns in a silicon wafer. On the spot region the silicon dioxide thickness is 140 nm and on the land it is 77 nm. The spot and the land have equal reflectance but opposite interferometric quadrature responses for protein layer. Protein is evenly immobilized on the entire chip and detected by reflectometry. Therefore there is no need for protein printing, nor spectrometers, nor high angles nor polarization control to image the surface-bound protein. The LC BioCD can facilitate research on protein microarrays.

Citation Download Citation

Xuefeng Wang, Ming Zhao, and David D. Nolte "A novel concept for protein microarray: land-contrast BioCD", Proc. SPIE 7170, Design and Quality for Biomedical Technologies II, 717003 (23 February 2009); https://doi.org/10.1117/12.809358

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