Instrumentation for simultaneous magnetic resonance and optical tomographic imaging of the rodent brain

James M. Masciotti; Jonghwan Lee; Mark Stewart; Andreas H. Hielscher

doi:10.1117/12.809882

20 February 2009 Instrumentation for simultaneous magnetic resonance and optical tomographic imaging of the rodent brain

James M. Masciotti, Jonghwan Lee, Mark Stewart, Andreas H. Hielscher

Author Affiliations +

Proceedings Volume 7171, Multimodal Biomedical Imaging IV; 71710Q (2009) https://doi.org/10.1117/12.809882
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

We present an instrument for simultaneous imaging of the rodent brain with frequency-domain optical tomography and magnetic resonance imaging. The instrument uses a custom-built fiber optic probe that allows for measurements in backreflectance geometry. The probe consists of 13 source and 26 detector fibers and is small enough to fit inside of a microMRI RF coil with an inner diameter of 38mm. Illumination by the source fibers is time demultiplexed by an optical fiber switch. A gain-modulated image intensifier CCD camera focuses onto the endpoints of large-core gradedindex detector fibers and collects the frequency-domain data. Imaging can be performed with source-modulation frequencies up to 1 GHz. The instrument is capable of acquiring multi-frequency optical tomography data at 2 wavelengths, and the data can be used to generate 3D maps of hemoglobin concentrations. At the same time magnetic resonance images can be acquired with in-plane resolution smaller than 100 micron. To illustrate the performance of the instrument we show results of small animal studies that involve inhalation of 100% carbogen and chemically induced seizures.

Citation Download Citation

James M. Masciotti, Jonghwan Lee, Mark Stewart, and Andreas H. Hielscher "Instrumentation for simultaneous magnetic resonance and optical tomographic imaging of the rodent brain", Proc. SPIE 7171, Multimodal Biomedical Imaging IV, 71710Q (20 February 2009); https://doi.org/10.1117/12.809882

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