Cellular imagery with total internal reflection holographic microscopy

William M. Ash III; Myung K. Kim

doi:10.1117/12.808029

12 February 2009 Cellular imagery with total internal reflection holographic microscopy

William M. Ash III, Myung K. Kim

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71820A (2009) https://doi.org/10.1117/12.808029
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Evanescent wave phase profiling is incorporated with digital holography into a new surface imaging technique termed total internal reflection holographic microscopy (TIRHM). Quantitative images of tissue structures and profiles of cellular membranes are presented to demonstrate the method's performance capabilities. Applications of this technique include measurements of cellular membranes and their transport processes without the addition of fluorophores. The angular spectrum method to compensate for tilt anamorphism due to the inclined TIR plane is also discussed.

Citation Download Citation

William M. Ash III and Myung K. Kim "Cellular imagery with total internal reflection holographic microscopy", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71820A (12 February 2009); https://doi.org/10.1117/12.808029

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