Red blood cell structure and dynamics explored with digital holographic microscopy

P. Marquet; B Rappaz; A. Barbul; R. Korenstein; C. Depeursinge; P. Magistretti

doi:10.1117/12.809224

23 February 2009 Red blood cell structure and dynamics explored with digital holographic microscopy

P. Marquet, B Rappaz, A. Barbul, R. Korenstein, C. Depeursinge, P. Magistretti

Proceedings Volume 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII; 71821A (2009) https://doi.org/10.1117/12.809224
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. Thanks to a decoupling procedure cell thickness and intracellular refractive index can be measured separately. Consequently, Mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), two highly relevant clinical parameters, have been measured non-invasively at a single cell level. The DHM nanometric axial and microsecond temporal sensitivities have permitted to measure the red blood cell membrane fluctuations (CMF) on the whole cell surface.

Citation Download Citation

P. Marquet, B Rappaz, A. Barbul, R. Korenstein, C. Depeursinge, and P. Magistretti "Red blood cell structure and dynamics explored with digital holographic microscopy", Proc. SPIE 7182, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 71821A (23 February 2009); https://doi.org/10.1117/12.809224

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