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Proceedings Article

Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation

[+] Author Affiliations
Yair Andegeko, Dmitry Pestov, Vadim V. Lozovoy, Marcos Dantus

Michigan State Univ. (USA)

Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830W (February 13, 2009); doi:10.1117/12.808429
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From Conference Volume 7183

  • Multiphoton Microscopy in the Biomedical Sciences IX
  • Ammasi Periasamy; Peter T. C. So
  • San Jose, CA | January 24, 2009

abstract

High-order dispersion of ultrashort laser pulses (with ~100 nm bandwidth) is shown to account for significant reduction of two-photon excitation fluorescence and second harmonic generation signal produced at the focal plane of a laser-scanning two-photon microscope. The second- and third-order corrections recover 20-40% of the signal intensity expected for a transform-limited laser pulse, while the rest depends on the proper compensation of higher-order terms. It can be accomplished through the use of a pulse shaper by measuring and correcting all nonlinear spectral phase distortions.

© (2009) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

Yair Andegeko ; Dmitry Pestov ; Vadim V. Lozovoy and Marcos Dantus
"Ultrafast multiphoton microscopy with high-order spectral phase distortion compensation", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71830W (February 13, 2009); doi:10.1117/12.808429; http://dx.doi.org/10.1117/12.808429


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