Polarization-resolved second-harmonic-generation imaging of photoaged dermal collagen fiber

Takeshi Yasui; Yu Takahashi; Tsutomu Araki

doi:10.1117/12.808672

13 February 2009 Polarization-resolved second-harmonic-generation imaging of photoaged dermal collagen fiber

Takeshi Yasui, Yu Takahashi, Tsutomu Araki

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71831X (2009) https://doi.org/10.1117/12.808672
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Polarization-resolved second-harmonic-generation (SHG) microscopy is useful for assessment of collagen fiber orientation in tissues. In this paper, we investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved SHG microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers whereas no-UVB-exposed skin was dominated by collagen orientation parallel to the meridian line of body. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging.

Citation Download Citation

Takeshi Yasui, Yu Takahashi, and Tsutomu Araki "Polarization-resolved second-harmonic-generation imaging of photoaged dermal collagen fiber", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831X (13 February 2009); https://doi.org/10.1117/12.808672

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available