Towards optimization and characterization of dye-embedded gold nanoparticle clusters for multiplexed optical imaging

M. A. McDonald; A. R. Hight Walker

doi:10.1117/12.814779

17 February 2009 Towards optimization and characterization of dye-embedded gold nanoparticle clusters for multiplexed optical imaging

M. A. McDonald, A. R. Hight Walker

Author Affiliations +

Proceedings Volume 7192, Plasmonics in Biology and Medicine VI; 71920L (2009) https://doi.org/10.1117/12.814779
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Metallic nanoparticle clusters coupling strong surface plasmons with a Raman reporter molecule have been developed for application in multiplexed optical imaging. Of interest to our work is the ability of the agents to serve as surface-enhanced Raman spectroscopy (SERS) probes. We present the seed-mediated synthesis and characterization of rhodamine B isothiocyante Au nanoparticle clusters (RhB-AuNPCs). RhB-AuNPCs are anisotropic structures which contain the Raman reporter, RhB, embedded between a gold aggregate core and gold surface layer. In contrast to typical SERS studies, the Raman signal originates from the probe (RhB-AuNPCs) and not from RhB incubated with a noble metal colloid. Characterization of the probes' optical properties is presented. The overall goal of our study is to prepare probes that may be used for the identification and spectroscopic labeling of multiple molecular biomarkers utilizing SERS imaging.

Citation Download Citation

M. A. McDonald and A. R. Hight Walker "Towards optimization and characterization of dye-embedded gold nanoparticle clusters for multiplexed optical imaging", Proc. SPIE 7192, Plasmonics in Biology and Medicine VI, 71920L (17 February 2009); https://doi.org/10.1117/12.814779

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