fDOT for in vivo follow-up of tumor development in mice lungs

Anne Koenig; Lionel Hervé; Anabela Da Silva; Jean-Marc Dinten; Jérôme Boutet; Michel Berger; Véronique Josserand; Jean-Luc Coll; Philippe Peltié; Philippe Rizo

doi:10.1117/12.727862

12 July 2007 fDOT for in vivo follow-up of tumor development in mice lungs

Anne Koenig, Lionel Hervé, Anabela Da Silva, Jean-Marc Dinten, Jérôme Boutet, Michel Berger, Véronique Josserand, Jean-Luc Coll, Philippe Peltié, Philippe Rizo

Author Affiliations +

Proceedings Volume 6629, Diffuse Optical Imaging of Tissue; 662915 (2007) https://doi.org/10.1117/12.727862
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

This paper presents in vivo experiments conducted on cancerous mice bearing mammary murine tumors. In order to reconstruct the fluorescence yield even in highly attenuating and heterogeneous regions like lungs, we developed a fDOT reconstruction method which at first corrects the light propagation model from optical heterogeneities by using the transmitted excitation light measurements. The same approach is also designed to enable working without immersing the mouse in adaptation liquid. The 3D fluorescence map is then reconstructed from the emitted signal of fluorescence and from the corrected propagation model by an ART (Algebraic Reconstruction Technique) algorithm. The system ability to reconstruct fluorescence distribution in presence of high attenuating objects has been validated on phantoms presenting a fluorescent absorbent inclusion. A study was conducted on mice to follow up lungs at different stages of tumor development. The mice were imaged after intravenous injection to the animal of a cancer specific fluorescent marker. A control experiment was conducted in parallel on healthy mice to ensure that the multiple injections of fluorophore did not induce parasite fluorescence distribution. These results validate our system performances for studying small animal lungs tumor evolution. Detection and localization of the fluorophore fixations expresses the tumor development.

Citation Download Citation

Anne Koenig, Lionel Hervé, Anabela Da Silva, Jean-Marc Dinten, Jérôme Boutet, Michel Berger, Véronique Josserand, Jean-Luc Coll, Philippe Peltié, and Philippe Rizo "fDOT for in vivo follow-up of tumor development in mice lungs", Proc. SPIE 6629, Diffuse Optical Imaging of Tissue, 662915 (12 July 2007); https://doi.org/10.1117/12.727862

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available