Two-photon microscopy of non-melanoma skin cancer: initial experience and diagnostic criteria ex vivo

Marica B. Ericson; John Paoli; Carl Ljungblad; Adaocha Odu; Maria Smedh; Ann-Marie Wennberg

doi:10.1117/12.725407

13 July 2007 Two-photon microscopy of non-melanoma skin cancer: initial experience and diagnostic criteria ex vivo

Marica B. Ericson, John Paoli, Carl Ljungblad, Adaocha Odu, Maria Smedh, Ann-Marie Wennberg

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 66300U (2007) https://doi.org/10.1117/12.725407
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Multiphoton microscopy is an interesting optical technique, which allows for non-invasive imaging of highly light scattering media such as human skin. Recent reports have showed the potential of applying this technique for 3D visualisation of cell structures of biological tissue without previous sectioning of the tissue samples. In this study, we have applied two-photon microscopy on excised lesions of human non-melanoma skin cancer ex vivo in order to find diagnostic criteria using this technique. The skin samples have been investigated by a multiphoton microscopy system based on a fs-pulsed Ti:sapphire laser connected to a confocal microscope. The autofluorescence of the skin was detected using excitation at 780 nm. The cell nuclei distribution turned out to be one important parameter, which can be used for discriminating between tumour and normal tissue. We are now developing a technique for automatic detection and characterisation of tissue, based on an image analysis algorithm. The detection of cell nuclei has been found crucial for this purpose. The goal is to develop a fast characterisation algorithm that can be used on line in connection to in vivo investigations. This would allow for a true non-invasive biopsy technique in the future.

Citation Download Citation

Marica B. Ericson, John Paoli, Carl Ljungblad, Adaocha Odu, Maria Smedh, and Ann-Marie Wennberg "Two-photon microscopy of non-melanoma skin cancer: initial experience and diagnostic criteria ex vivo", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300U (13 July 2007); https://doi.org/10.1117/12.725407

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available