Intravital multiphoton microscopy for imaging hepatobiliary function

Feng-Chieh Li; Tzu-Lin Sun; Hsuan-Shu Lee; Shu-Mei Yang; Chen-Yuan Dong

doi:10.1117/12.728079

13 July 2007 Intravital multiphoton microscopy for imaging hepatobiliary function

Feng-Chieh Li, Tzu-Lin Sun, Hsuan-Shu Lee, Shu-Mei Yang, Chen-Yuan Dong

Proceedings Volume 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III; 663014 (2007) https://doi.org/10.1117/12.728079
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Liver is the chemical factory in body responsible for important functions such as metabolism and detoxification. When liver can not be regenerated in time to amend damages that has occurred, failure of hepatic functions can result. Traditionally, the study of liver pathology has depended on histological techniques, but such methods are limited to ex-vivo observation. In order to study hepatic metabolism in vivo, we have designed a hepatic imaging chamber made of biocompatible titanium alloy (6V4Al-Ti, ELI grade). In combination with multiphoton and second harmonic generation microscopy, our approach allows the intravital observation of hepatic intravital activities to be achieved. Processes such as hepatic metabolism and disease progression can be studied using this methodology.

Citation Download Citation

Feng-Chieh Li, Tzu-Lin Sun, Hsuan-Shu Lee, Shu-Mei Yang, and Chen-Yuan Dong "Intravital multiphoton microscopy for imaging hepatobiliary function", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663014 (13 July 2007); https://doi.org/10.1117/12.728079

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