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Proceedings Article

Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy

[+] Author Affiliations
H. B. Manning, D. M. Owen, E. Auksorius, P. A. A. de Beule, S. Oddos, C. B. Talbot, C. Dunsby, I. Munro, A. I. Magee, M. A. A. Neil, P. M. W. French

Imperial College London (United Kingdom)

Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663019 (July 13, 2007); doi:10.1117/12.728479
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From Conference Volume 6630

  • Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
  • Tony Wilson; Ammasi Periasamy
  • Munich, Germany | June 17, 2007

abstract

We describe the characterisation of a hyperspectral fluorescence lifetime imaging microscope that exploits high-speed time-gated imaging technology and a tunable continuum source for 6-D fluorescence imaging. This line-scanning confocal microscope can record the full spectral-temporal (i.e. excitation-emission-lifetime) fluorescence matrix at each pixel in a three dimensional (x-y-z) sample. This instrument has been applied to biological samples including model membranes and live cells labelled with the phase-sensitive membrane dye di-4-ANEPPDHQ, for which significant variation of lifetime with emission wavelength is observed.

© (2007) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Citation

H. B. Manning ; D. M. Owen ; E. Auksorius ; P. A. A. de Beule ; S. Oddos, et al.
"Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy", Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 663019 (July 13, 2007); doi:10.1117/12.728479; http://dx.doi.org/10.1117/12.728479


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