Techniques and applications of digital holographic microscopy for life cell imaging

Björn Kemper; Patrik Langehanenberg; Ilona Bredebusch; Jürgen Schnekenburger; Gert von Bally

doi:10.1117/12.728262

13 July 2007 Techniques and applications of digital holographic microscopy for life cell imaging

Björn Kemper, Patrik Langehanenberg, Ilona Bredebusch, Jürgen Schnekenburger, Gert von Bally

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66330D (2007) https://doi.org/10.1117/12.728262
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

In connection with microscopy, digital holography provides contact-less, marker-free, quantitative phase-contrast imaging. Particularly, the feature of (subsequent) numerical auto focus adjustment enables applications in the field of life cell analysis. Here, prospects for long term time-lapse investigations in toxicology and cancer research as well as for monitoring of fast dynamic processes like shape variations are opened up. The evaluation of the obtained quantitative phase contrast provides data for thickness monitoring as well as for the observation of cell swelling kinetics due to osmotic stimulation. Results from investigations on living cells demonstrate digital holographic microscopy application fields for quantitative life cell analytics.

Citation Download Citation

Björn Kemper, Patrik Langehanenberg, Ilona Bredebusch, Jürgen Schnekenburger, and Gert von Bally "Techniques and applications of digital holographic microscopy for life cell imaging", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330D (13 July 2007); https://doi.org/10.1117/12.728262

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